Negative stain EM of IgG

Image of Cryo-EM tomography

Cryo-EM slice of fruit fly cell mitochondria

Photo of the Krios in the factory

UW-Madison Krios at the factory in Eindhoven

Photo of members of the Cryo-EM team

Teaching students about cryo-EM

Frozen-hydrated β-galactosidase

Cryo-EM grid square map

Cryo-EM grid low magnification montage

Apoferritin at 1.9 Angstrom

Cryo-FIB milled yeast

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a research facility located in the Hector F. DeLuca Biochemical Sciences Complex on the University of Wisconsin-Madison campus. The UW-Madison CEMRC is dedicated to providing instrumentation, technical assistance, training, and access to cryo-EM for the UW-Madison research community. When fully operational, the CEMRC will operate four Thermo Scientific cryo-microscopes for data collection by single particle, tomography, and micro-ED. The microscopes are overseen by experienced staff who offer consultation and training in negative-stain and vitrified sample preparation, single particle analysis, tomography, data processing and additional computational support.

The UW-Madison CEMRC welcomes investigators from other universities and industry.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a cross-campus initiative led by a coalition of partners including the Department of Biochemistry, the School of Medicine and Public Health, the Morgridge Institute for Research, the UW Carbone Cancer Center, the Office of the Vice Chancellor for Research and Graduate Education, and the College of Engineering Nanoscale Imaging and Analysis Center.

Cryo-EM @ UW

Project development

If you are interested in starting a project, please fill out and email us the project and sample submission forms. We will then schedule a time with you to meet and discuss your project.

CEMRC_ProjectForm

CEMRC_SampleSubmissionForm

The CEMRC uses iLab to track your projects.

To log into your iLab account with your netID, use the following link. https://uwmadison.ilabsolutions.com

To access and schedule equipment time and staff support on CEMRC iLab calendars, please refer to this guide. iLab_access_calendars

Questions?

Please email us at:

emontemayor@wisc.edu or erwright2@wisc.edu

Updates from the director