Negative stain EM of IgG

Frozen-hydrated β-galactosidase

Apoferritin at 1.9 Angstrom

Nano-scale Bucky Badgers!

Cryo-FIB milled HeLa cell with slice from 3D tomogram.

SARS-CoV-2 spike.

Dr. Jae Yang loading cryo-samples into the Aquilos cryo-FIB-SEM.

Cryo-CLEM workflow to study mitochondria structure in virus-infected cells.

Polymer coated single-walled nanotubes. Imaging by Dr. Kai Cai. Sample from Andrews lab, UW-Madison.

Internal architecture of a neuron.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a research facility located in the Hector F. DeLuca Biochemical Sciences Complex on the University of Wisconsin-Madison campus. The UW-Madison CEMRC is dedicated to providing instrumentation, technical assistance, training, and access to cryo-EM for the UW-Madison research community. The CEMRC manages and operates four cryo-microscopes for data collection by single particle, tomography, and micro-ED. The microscopes are overseen by experienced staff who offer consultation and training in negative-stain and vitrified sample preparation, single particle analysis, tomography, data processing and additional computational support.

The UW-Madison CEMRC welcomes investigators from other universities and industry.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a cross-campus initiative led by a coalition of partners including the Department of Biochemistry, the School of Medicine and Public Health, the Morgridge Institute for Research, the UW Carbone Cancer Center, the Office of the Vice Chancellor for Research and Graduate Education, and the College of Engineering Nanoscale Imaging and Analysis Center.

Cryo-EM @ UW

  • New Operations Manager for CEMRC and MCCET- Bryan Sibert

    Congratulations to Bryan Sibert, PhD on being promoted to Operations Manager for the facilities! Bryan will be our new Operations Manager. Contact him at sibert@wisc.edu to say congrats! People

  • We are looking for a new CryoEM Scientist-it could be you!

    The University of Wisconsin-Madison Cryo-EM Research Center (CEMRC) and the NIH funded Midwest Center for Cryo-Electron Tomography (MCCET) are seeking a Cryo-EM Scientist to support operations of our expanding cryo-electron microscopy facilities.  This premier research …

  • Equipment upgrades to the Titan Krios G3i

    The UW-Madison Cryo-EM Research Center Krios G3i 300 kV TEM will be undergoing upgrades! The G3i Krios will be updated with new software, fringe-free imaging (FFI) for high-speed single particle and tomography data collection, and …

  • Congratulations to our award-winning staff and students

    Congrats to Jae Yang, PhD for her Professional Technical Staff Award at the M&M 2022 conference. Montage cryo-electron tomography: a high throughput and flexible data collection scheme to explore in-situ cellular landscapes while preserving high-resolution …

  • Congressional Staff Tour Cryo-EM Facilities

    In August the Cryo-EM Facility was visited by Wisconsin Congressional Staffers from 9 congressional offices, including those of Senator Tammy Baldwin and Senator Ron Johnson.  Thank you to all who worked so hard to make that …

  • Smashing success for the 42nd Steenbock Symposium June 7th – 8th 2022

    We celebrated the official opening of the UW-Madison Cryo-EM Research Center (CEMRC) and the NIH-funded National Center, the Midwest Center for Cryo-Electron Tomography (MCCET), at the 42nd Steenbock Symposium, “Opening the Doors to Cryo-EM” hosted …

  • More News

Project development

If you are interested in starting a project, please fill out and email us the project and sample submission forms. We will then schedule a time with you to meet and discuss your project.

CEMRC_ProjectForm

CEMRC_SampleSubmissionForm

Ready for data collection on the Arctica or Krios? Please please fill out and email us a pre-session information form.

CEMRC_PreSessionInfo

The CEMRC uses iLab to track your projects.

To log into your iLab account with your netID, use the following link. https://uwmadison.ilabsolutions.com

To access and schedule equipment time and staff support on CEMRC iLab calendars, please refer to this guide. iLab_access_calendars

To access your imaging data using Globus, please refer to this guide.
CEMRC_GlobusDocumentation

Questions?

Please email us at:

cryoEM@biochem.wisc.edu, General Information

Elizabeth Wright, Director
Matthew Larson, Systems Administrator
Alex Cotten, Administrative Assistant

Office hours: By appointment only- email cryoem@biochem.wisc.edu

Shipping Address:

Cryo-EM Research Center
University of Wisconsin-Madison
440 Henry Mall, Rm. 1220
Madison, WI 53706

Updates from the director

Acknowledgements.

Any use of the resources in the UW-Madison Cryo-EM Research Center (CEMRC) or the NIH-funded Midwest Center for Cryo-ET (MCCET) requires acknowledgment of use of the resources in presentations and publications as follows:

Standard Acknowledgement:
CEMRC: “Some of this work was performed in the Cryo-EM Research Center (CEMRC) in the Department of Biochemistry at the University of Wisconsin-Madison.”

MCCET: “Some of this work was performed at the Midwest Center for Cryo-ET (MCCET) and the Cryo-EM Research Center located in the Department of Biochemistry at the University of Wisconsin-Madison, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM139168).”

Authorship:
We perform work that is highly collaborative. For each project, our center group members devote significant effort in carrying out the projects at one or more phases in the project pipeline from specimen preparation and data collection to structure interpretation. If your research project involved significant contributions from CEMRC or MCCET staff, please recognize our staff with co-authorship on presentations and publications.