Negative stain EM of IgG

Frozen-hydrated β-galactosidase

Apoferritin at 1.9 Angstrom

Nano-scale Bucky Badgers!

Cryo-FIB milled HeLa cell with slice from 3D tomogram.

SARS-CoV-2 spike.

Dr. Jae Yang loading cryo-samples into the Aquilos cryo-FIB-SEM.

Cryo-CLEM workflow to study mitochondria structure in virus-infected cells.

Polymer coated single-walled nanotubes. Imaging by Dr. Kai Cai. Sample from Andrews lab, UW-Madison.

Internal architecture of a neuron.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a research facility located in the Hector F. DeLuca Biochemical Sciences Complex on the University of Wisconsin-Madison campus. The UW-Madison CEMRC is dedicated to providing instrumentation, technical assistance, training, and access to cryo-EM for the UW-Madison research community. The CEMRC manages and operates four cryo-microscopes for data collection by single particle, tomography, and micro-ED. The microscopes are overseen by experienced staff who offer consultation and training in negative-stain and vitrified sample preparation, single particle analysis, tomography, data processing and additional computational support.

The UW-Madison CEMRC welcomes investigators from other universities and industry.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a cross-campus initiative led by a coalition of partners including the Department of Biochemistry, the School of Medicine and Public Health, the Morgridge Institute for Research, the UW Carbone Cancer Center, the Office of the Vice Chancellor for Research and Graduate Education, and the College of Engineering Nanoscale Imaging and Analysis Center.

Cryo-EM @ UW

  • The Cryo-EM Centers at UW-Madison are hosting a grand opening!

    Mark your calendars and save the date! Opening Doors to Cryo-EM 42nd Steenbock Symposium – June 7, 2022 to June 8, 2022 Discovery Building Grand Opening of the Cryo-Electron Microscopy Research Center & the Midwest Center …

  • The EMDB is hiring a Resource Manager!

    The EMDB, located in Hinxton, UK, is looking to recruit an EMDB Resource Manager to lead the activities surrounding the EMDB archive (, one of the three core wwPDB archives and one of the five …

  • Cryo-EM Research Center Office Hours

    The Cryo-EM Research Center will hold office hours every Thursday morning at 9 am on Zoom. For more information, please see the attached flier or e-mail for more information. CEMRC_OfficeHours

  • Faculty Call: Assistant, Associate, or Full Professor of Biochemistry

    The Department of Biochemistry ( at the University of Wisconsin-Madison invites applications for a new faculty position to build on its strength in biochemical research. This position is central to our multi-year hiring plan in …

  • Faculty Call: Virologist Working on RNA Biology — Cluster Hire

    The University of Wisconsin-Madison Institute for Molecular Virology (IMV), in collaboration with the Departments of Pathobiological Sciences, Medical Microbiology & Immunology, or Biochemistry, seek to hire a tenure-track Assistant Professor or Associate Professor. The faculty …

  • Faculty Call at The Medical College of Wisconsin (Cryo-EM Structural Biology)

    The Medical College of Wisconsin (MCW) Cancer Center (CC) in partnership with basic science departments (Biochemistry, Biophysics, Pharmacology & Toxicology, Cell Biology, Neuroscience and Anatomy, Microbiology & Immunology) have committed to expand strengths in Structural …

  • More News

Project development

If you are interested in starting a project, please fill out and email us the project and sample submission forms. We will then schedule a time with you to meet and discuss your project.



Ready for data collection on the Arctica or Krios? Please please fill out and email us a pre-session information form.


The CEMRC uses iLab to track your projects.

To log into your iLab account with your netID, use the following link.

To access and schedule equipment time and staff support on CEMRC iLab calendars, please refer to this guide. iLab_access_calendars

To access your imaging data using Globus, please refer to this guide.


Please email us at:, General Information

Elizabeth Wright, Director
Matthew Larson, Systems Administrator
Alex Cotten, Administrative Assistant

Office hours: Thursdays at 9:00 am Central Time via Zoom. Walk-up appointments available upon request.

Shipping Address:

Cryo-EM Research Center
University of Wisconsin-Madison
440 Henry Mall, Rm. 1220
Madison, WI 53706

Updates from the director


Any use of the resources in the UW-Madison Cryo-EM Research Center (CEMRC) or the NIH-funded Midwest Center for Cryo-ET (MCCET) requires acknowledgment of use of the resources in presentations and publications as follows:

Standard Acknowledgement:
CEMRC: “Some of this work was performed in the Cryo-EM Research Center (CEMRC) in the Department of Biochemistry at the University of Wisconsin-Madison.”

MCCET: “Some of this work was performed at the Midwest Center for Cryo-ET (MCCET) and the Cryo-EM Research Center located in the Department of Biochemistry at the University of Wisconsin-Madison, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM139168).”

We perform work that is highly collaborative. For each project, our center group members devote significant effort in carrying out the projects at one or more phases in the project pipeline from specimen preparation and data collection to structure interpretation. If your research project involved significant contributions from CEMRC or MCCET staff, please recognize our staff with co-authorship on presentations and publications.