Negative stain EM of IgG

Frozen-hydrated β-galactosidase

Apoferritin at 1.9 Angstrom

Nano-scale Bucky Badgers!

Cryo-FIB milled HeLa cell with slice from 3D tomogram.

SARS-CoV-2 spike.

Dr. Jae Yang loading cryo-samples into the Aquilos cryo-FIB-SEM.

Cryo-CLEM workflow to study mitochondria structure in virus-infected cells.

Polymer coated single-walled nanotubes. Imaging by Dr. Kai Cai. Sample from Andrews lab, UW-Madison.

Internal architecture of a neuron.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a research facility located in the Hector F. DeLuca Biochemical Sciences Complex on the University of Wisconsin-Madison campus. The UW-Madison CEMRC is dedicated to providing instrumentation, technical assistance, training, and access to cryo-EM for the UW-Madison research community. The CEMRC manages and operates four cryo-microscopes for data collection by single particle, tomography, and micro-ED. The microscopes are overseen by experienced staff who offer consultation and training in negative-stain and vitrified sample preparation, single particle analysis, tomography, data processing and additional computational support.

The UW-Madison CEMRC welcomes investigators from other universities and industry.

The UW-Madison Cryo-Electron Microscopy Research Center (CEMRC) is a cross-campus initiative led by a coalition of partners including the Department of Biochemistry, the School of Medicine and Public Health, the Morgridge Institute for Research, the UW Carbone Cancer Center, the Office of the Vice Chancellor for Research and Graduate Education, and the College of Engineering Nanoscale Imaging and Analysis Center.

PROJECT DEVELOPMENT

If you’re interested in starting a project, please complete and email us the Project and Sample Submission Forms. Once we receive your forms, we’ll coordinate a convenient time to meet and discuss your project in detail. During this meeting, we’ll explore your ideas, requirements, and goals to ensure we provide the best possible support for your project.

Project Form

Sample Submission Form

Data Collection

Ready for data collection on the Arctica or Krios? Please complete and email us a Pre-session Information Form.

Pre-session Information Form

iLab Login

The CEMRC utilizes iLab to efficiently manage and track all projects. Log into your iLab account with your NetID.

iLab Login

GUIDES

iLab Access Calendar Guide

To access and schedule equipment time and staff support on CEMRC iLab calendars, please refer to this guide.

iLab Access Calendar

Accessing Your Imaged Data via Globus

To access and manage your imaging data using Globus, please follow the steps outlined in this guide.

Using Globus

ACKNOWLEDGEMENTS

Any use of the resources in the UW-Madison Cryo-EM Research Center (CEMRC) or the NIH-funded Midwest Center for Cryo-ET (MCCET) requires acknowledgement of use of the resources in presentations and publications as follows:

 

STANDARD ACKNOWLEDGEMENT
CEMRC: “Some of this work was performed in the Cryo-EM Research Center (CEMRC) in the Department of Biochemistry at the University of Wisconsin-Madison.”

MCCET: “Some of this work was performed at the Midwest Center for Cryo-ET (MCCET) and the Cryo-EM Research Center located in the Department of Biochemistry at the University of Wisconsin-Madison, supported by the NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program (U24 GM139168).”

AUTHORSHIP
We perform work that is highly collaborative. For each project, our center group members devote significant effort in carrying out the projects at one or more phases in the project pipeline, from specimen preparation and data collection to structure interpretation. If your research project involved significant contributions from CEMRC or MCCET staff, please recognize our staff with co-authorship on presentations and publications.

GENERAL INFORMATION

CEMRC OFFICE HOURS


THURSDAYS
9:00 AM – 10 AM

DeLuca Biochemistry Building
B1131, 420 Henry Mall

Visit us for all inquiries regarding training, services, and more.

Questions?

Whether it’s about our services, projects, or anything else, don’t hesitate to get in touch.

Cryo-EM Email

Stay Connected

If you’d like to stay updated with our latest news and events, sign up to receive email notifications.

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Contact Information


Elizabeth Wright, Director

Bryan Sibert, Operations Manager

Matthew Larson, Systems Administrator

Corina Evans, Administrative Assistant II

Shipping Address


Cryo-EM Research Center
University of Wisconsin-Madison
420 Henry Mall, Rm. B1131
Madison, WI 53706