The UW–Madison Cryo-EM Research Center offers numerous services and training opportunities.
General Office Hours.
By appointment only. Please contact cryoem@biochem.wisc.edu to schedule.
Rates.
The facility is open for assisted or independent use. The rates for the various services and materials are below. Rates approved October 2020.
TRAINING, SERVICES, OR ASSISTED USE |
INTERNAL ACADEMIC |
EXTERNAL ACADEMIC(NON-PROFIT) |
INDUSTRY |
| Staff labor | $30 / hour | $47 / hour | $93 / hour |
| Negative stain grid prep. | $57 / hour | $89 / hour | $178 / hour |
| Immuno/negative stain grid prep. | $126 / hour | $196 / hour | $392/ hour |
| Cryo grid preparation | $48 / hour | $76 / hour | $150 / hour |
| ACE600 (glow discharge) | $40 / hour | $63 / hour | $124 / hour |
| ACE600 (Carbon or Platinum coating) | $62 / hour | $96 / hour | $192 / hour |
| GloQube (glow discharge) | $37 / hour | $58 / hour | $115 / hour |
| Vitrobot (plunge freezing) | $75 / hour | $117 / hour | $233 / hour |
| Talos L120 (TEM) | $80 / hour | $124 / hour | $248 / hour |
| Talos L120 (Cryo-TEM)* | $62 / hour * | $96 / hour * | $193 / hour * |
| Talos Arctica (Cryo-TEM)* | $1,300 / day * | $2,015 / day * | $4,031 / day * |
| Titan Krios (Cryo-TEM)* | $1,515 / day * | $2,348 / day * | $4,696 / day * |
| Aquilos (Cryo-FIB-SEM)* | $497 / day * | $771 / day * | $1,542 / day * |
*plus staff hourly labor as needed
INDEPENDENT USE |
INTERNAL ACADEMIC |
EXTERNAL ACADEMIC(NON-PROFIT) |
INDUSTRY |
| Negative stain grid prep. | $27 / hour | $42 / hour | $85 / hour |
| Immuno/negative stain grid prep. | $96 / hour | $149 / hour | $299 / hour |
| Cryo grid preparation | $18 / hour | $29 / hour | $57 / hour |
| ACE600 (glow discharge) | $10 / hour | $16 / hour | $31 / hour |
| ACE600 (Carbon or Platinum coating) | $32 / hour | $49 / hour | $99 / hour |
| GloQube (glow discharge) | $7 / hour | $11 / hour | $22 / hour |
| Vitrobot (plunge freezing) | $45 / hour | $70 / hour | $140 / hour |
| Talos L120 (TEM) | $50 / hour | $77 / hour | $155 / hour |
Available supplies and materials. |
Cost per unit |
| EM starter kit (tweezers, boxes, crossgrating standard) | $160 |
| Negative Stain Grids | $2.25 |
| Negative Stain Grid Box | $5 |
| Cryo-EM Grid Box | $10 |
| Cryo-EM Grids: C-Flat Copper, 200 Mesh — 2/2 | $8 |
| Cryo-EM Grids: C-Flat Copper, 200 Mesh — 4/2 | $8 |
| Cryo-EM Grids: C-Flat Copper, 300 Mesh — 2/1 | $8 |
| Cryo-EM Grids: C-Flat Copper, 300 Mesh — 4/1 | $8 |
| Cryo-EM Grids: C-Flat Copper, 400 Mesh — 1.2/1.3 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 200 Mesh — 1.2/1.3 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 200 Mesh — 2/2 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 200 Mesh — 3.5/1 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 300 Mesh — 1/2 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 300 Mesh — 1.2/1.3* | $10 |
| Cryo-EM Grids: Quantifoil Copper, 300 Mesh — 2/1 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 300 Mesh — 7/2 | $8 |
| Cryo-EM Grids: Quantifoil Copper, 400 Mesh — 1.2/1.3 | $8 |
| Cryo-EM Grids: UltaAufoil, 300 Mesh — 1.2/1.3 | $15 |
| Cryo-EM Grids: UltaAufoil, 200 Mesh — 2/2 | $15 |