This workshop will help you learn the fundamentals of cryo-electron tomography (cryo-ET) including sample preparation, FIB-milling, correlative imaging, and cryo-ET data collection and analysis. Whether you have been collecting single-particle cryo-EM data and are now looking to start your first tomography project, or have experience with tomography, you’ll come away with fresh ideas and strategies you can put to use right away. The morning sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only. Connect face-to-face with colleagues and build community with other tomography researchers at the Midwest Center for Cryo-Electron Tomography (MCCET) at the University of Wisconsin – Madison.
Images courtesy of Jae Yang, PhD and Veronika Vrbovská, PhD
Thank you everyone for attending the workshop in-person and remotely!
Post-Workshop Gallery of Images
Final Schedule
View all the recorded morning sessions at the MCCET – Bucky Cryo YouTube Channel.
Topics include:
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- Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.
- Training in data acquisition workflows that include screening, data collection, flexible scripting, and basic data processing.
- Afternoon sessions with specimen preparation methods including PRIMO micro-patterning, plunge freezing, high-pressure freezing, and other novel approaches.
- Instruction in 2D/3D correlative imaging workflows using a cryo-confocal microscope and the integrated fluorescence module in the Aquilos 2.
- Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods.
WHEN:
Monday April 17, 2023 to Thursday April 20, 2023
Breakfast begins at 8:00 a.m. followed by the morning program from 9 a.m. to 12:00 p.m.
Afternoon breakout sessions for hands-on use of instrumentation until 5:00 p.m.
Workshop attendance no-cost, hotel and travel costs not-included.
Meals are included.
WHERE:
HF Deluca Biochemistry Building, University of Wisconsin, Madison
Note: In-person space is limited. Registration closes March 3rd 2023
Morning sessions will be recorded for future viewing.
Hotel and Travel Information
Union South is located within 2-blocks of the MCCET facility, and has a reserved block of rooms.
More information to come on room reservation information.
Union South, 1308 W. Dayton St.
About Madison
Visiting the city of Madison, Wisconsin
Contact Alex Cotten cotten@wisc.edu or Jacqui van Twest vantwest@wisc.edu with any questions!
Speakers and organizers:
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Prof. Elizabeth Wright, University of Wisconsin-Madison, Director of Midwest Center for Cryo-Electron Tomography |
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Prof. Marisa Otegui, University of Wisconsin-Madison, Center for Quantitative Cell Imaging at UW-Madison |
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Prof. Alberto Bartesaghi, Duke University School of Medicine and Duke Institute for Brain Sciences |
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Prof. Lauren Ann Metskas, Biological Sciences and Chemistry at Purdue University |
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Zunlong Ke, PhD Postdoctoral fellow at the Max Planck Institute of Biochemistry |
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Daija Bobe, Scientist at the National Center for In-Situ Tomographic Ultramicroscopy (Virtual Talk) |
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Alex Cotten, Administrative Assistant at the Midwest Center for Cryo-Electron Tomography – organizer and facilitator contact |
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Jacqui van Twest, Project Manager at the Midwest Center for Cryo-Electron Tomography – organizer and facilitator contact |
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Bryan Sibert, PhD, Operations Manager at the Midwest Center for Cryo-Electron Tomography |
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Matt Larson, PhD, Systems Administer at the Midwest Center for Cryo-Electron Tomography |
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Jae Yang, PhD, Research Scientist at the Midwest Center for Cryo-Electron Tomography |
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Sergey Suslov, PhD, ThermoFisher Scientific Application Specialist |
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Veronika Vrbovská, PhD, ThermoFisher Scientific Application Specialist |
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Alex Hall, PhD, Product Application Specialist, Materials & Structural Analysis with ThermoFisher Scientific |