We celebrated the official opening of the UW-Madison Cryo-EM Research Center (CEMRC) and the NIH-funded National Center, the Midwest Center for Cryo-Electron Tomography (MCCET), at the 42nd Steenbock Symposium, “Opening the Doors to Cryo-EM” hosted by the UW-Madison Department of Biochemistry. The gathering brought more than 400 members of the Cryo-EM community together from UW Madison and across the globe and included talks, poster sessions, networking, tours of facilities and workshops!